Journal
NATURE METHODS
Volume 17, Issue 5, Pages 505-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-020-0821-0
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Funding
- EPSRC [EP/R029407/1] Funding Source: UKRI
- MRC [MR/N020413/1] Funding Source: UKRI
- European Research Council [695511] Funding Source: Medline
- Medical Research Council [MR/N020413/1] Funding Source: Medline
- Wellcome Trust [104633] Funding Source: Medline
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'Nativeomics' enables identification of ligands bound to membrane proteins through detection of intact protein-ligand assemblies followed by dissociation and identification of individual ligands within the same mass spectrometry experiment. Ligands bound to protein assemblies provide critical information for function, yet are often difficult to capture and define. Here we develop a top-down method, 'nativeomics', unifying 'omics' (lipidomics, proteomics, metabolomics) analysis with native mass spectrometry to identify ligands bound to membrane protein assemblies. By maintaining the link between proteins and ligands, we define the lipidome/metabolome in contact with membrane porins and a mitochondrial translocator to discover potential regulators of protein function.
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