Journal
NATURE BIOTECHNOLOGY
Volume 38, Issue 7, Pages 856-U10Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41587-020-0527-y
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Funding
- National Key R&D Program of China [2019YFA0110802, 2019YFA0802800]
- National Natural Science Foundation of China [81670470, 81873685, 31925011, 91940306]
- Shanghai Municipal Commission for Science and Technology [18411953500]
- Innovation Program of the Shanghai Municipal Education Commission [2019-01-07-00-05-E00054]
- ECNU Public Platform for Innovation [011]
- Fundamental Research Funds for the Central Universities
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Base editors that modify both adenine and cytosine broaden the potential applications of base editing. Although base editors are useful tools for precise genome editing, current base editors can only convert either adenines or cytosines. We developed a dual adenine and cytosine base editor (A&C-BEmax) by fusing both deaminases with a Cas9 nickase to achieve C-to-T and A-to-G conversions at the same target site. Compared to single base editors, A&C-BEmax's activity on adenines is slightly reduced, whereas activity on cytosines is higher and RNA off-target activity is substantially decreased.
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