Journal
NATURE BIOTECHNOLOGY
Volume 38, Issue 5, Pages 582-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41587-020-0455-x
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Funding
- National Natural Science Foundation of China [31788103]
- Strategic Priority Research Program of the Chinese Academy of Sciences (Precision Seed Design and Breeding) [XDA24000000]
- National Key Research and Development Program of China [2016YFD0101804]
- US National Institutes of Health (NIH) [U01AI142756, RM1HG009490, R01EB022376, R35GM118062]
- Howard Hughes Medical Institute (HHMI)
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Prime editors are optimized for use in plants. Prime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8%.
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