Two new diterpenoids with their antiproliferative activities from the supercritical fluid extraction of Croton crassifolius root

Supercritical fluid extraction was applied to obtain the lower polarity extracts from Croton crassifolius roots, and chemical investigation of which led to the isolation and identification of two new diterpenoids, named crassifolius P (1) and crassifolius Q (2). In vitro anti-proliferative activities of compounds 1 and 2 on A549, Hep-G2 and Hela tumor cell lines were evaluated. The two new compounds exhibited obvious selectivity to tumor cells with IC50 values ranging from 20.43 +/- 1.18 mu M to 25.72 +/- 1.32 mu M.

Automated summary

Supercritical fluid extraction was used to obtain lower polarity extracts from Croton crassifolius roots, resulting in the isolation and identification of two new diterpenoids, crassifolius P (1) and crassifolius Q (2). The two compounds showed significant selectivity to tumor cells with IC50 values ranging from 20.43 +/- 1.18 μM to 25.72 +/- 1.32 μM.