Journal
NATURAL PRODUCT RESEARCH
Volume 35, Issue 21, Pages 4169-4172Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2020.1744136
Keywords
linamarin; Cassava; liquid nitrogen; cryocooling; cytotoxicity
Categories
Funding
- Ministry of Science Technology and Research, Sri Lanka
Ask authors/readers for more resources
A novel cryocooled extraction method was developed to isolate linamarin from cassava root peel, resulting in the highest reported yield to date. The cytotoxicity of cassava crude extract was found to be higher than pure linamarin in the comprehensive cytotoxic assay using multiple cell lines, with results comparable to other studies in the literature.
During the last three decades, studies of linamarin extracted from cassava have received increased attention due to the presence of high cyanogenic compounds in these extracts. The methods that are utilized to isolate linamarin are either tedious or use acidic conditions resulting in poor yields. In this study, a novel cryocooled method of extraction has been developed to isolate linamarin from Cassava root peel. Approximately 18 g of linamarin was isolated from 1 kg of fresh Cassava root peel, which is the highest amount reported to date. Linamarin was fully characterized using NMR, IR and LCMS. The anti-cancer properties of pure linamarin and Cassava crude extract were evaluated by a comprehensive cytotoxic assay, using MCF-7, HepG2, NCI H-292, AN3CA and MRC-5 cell lines. The crude extract showed higher cytotoxicity compared to pure linamarin. The results of the biological evaluation are comparable to other reported studies in the literature.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available