Journal
NANOTECHNOLOGY
Volume 31, Issue 36, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1361-6528/ab95b6
Keywords
liquid-core nanocapsules; ratiometric fluorescent thermometers; intracellular temperature
Funding
- National Key R&D Program of China [2017YFE0196400, 2016YFA0200800]
- NSFC [51672284, 21975269]
- Beijing Natural Science Foundation [2181002]
- Chinese Academy of Sciences [1A1111KYSB20180017, QYZDJ-SSW-JSC032, XDB17000000]
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Intracellular thermometry with favorable biocompatibility and precision is essential for insight into temperature-related cellular events. Here, liquid-core nanocapsule ratiometric fluorescent thermometers (LCN-RFTs) were prepared by encapsulating thermosensitive organic fluorophores (N,N'-di(2-ethylhexyl)-3,4,9,10-perylene tetracarboxylic diimide, DEH-PDI) with hydrophobic solvent (2,2,4-trimethylpentane, TMP) into polystyrene/silica hybrid nanoshells. As the fluorescent thermosensitive unit of the LCN-RFT, the TMP solution of DEH-PDI was responsible for the fluorescence response to temperature. Benefitting from the hydrophilic nanoshells, the LCN-RFTs exhibited favorable anti-interference and biocompatibility. Furthermore, the LCN-RFTs showed an excellent precision of 0.02 degrees C-0.10 degrees C in a simulated physiological environment from 10.00 degrees C to 90.00 degrees C, and were employed to realize intracellular thermometry with an outstanding precision of 0.06 degrees C-0.14 degrees C. This work provides a feasible method of using hydrophobic organic fluorophores for intracellular thermometry by encapsulating them into nanocapsules.
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