Journal
NANO LETTERS
Volume 20, Issue 7, Pages 4775-4781Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.0c00448
Keywords
SIM; super-resolution; nonlinear; NIR; upconversion nanoparticles; deep tissue
Categories
Funding
- Australian Research Council Discovery Projects [DP190101058, DP200101970]
- Sydney Research Accelerator prize
- Shenzhen S c i e n c e and Technology Program [KQTD20170810110913065]
- Australia China Science and Research Fund Joint Research Centre for POCT [ACSRF65827]
- Australian Research Council DECRA fellowships [DE200100074, DE190100609]
- China Scholarship Council [201706020170, 201607950009, 201706170028, 201508530231, 201708200004]
Ask authors/readers for more resources
Video-rate super-resolution imaging through biological tissue can visualize and track biomolecule interplays and transportations inside cellular organisms. Structured illumination microscopy allows for wide-field super resolution observation of biological samples but is limited by the strong extinction of light by biological tissues, which restricts the imaging depth and degrades its imaging resolution. Here we report a photon upconversion scheme using lanthanide-doped nanoparticles for wide-field super-resolution imaging through the biological transparent window, featured by near-infrared and low-irradiance nonlinear structured illumination. We demonstrate that the 976 nm excitation and 800 nm upconverted emission can mitigate the aberration. We found that the nonlinear response of upconversion emissions from single nanoparticles can effectively generate the required high spatial frequency components in the Fourier domain. These strategies lead to a new modality in microscopy with a resolution below 131 nm, 1/7th of the excitation wavelength, and an imaging rate of 1 Hz.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available