Journal
MOLECULES
Volume 25, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/molecules25102396
Keywords
anthocyanins; Vitis coignetiae Pulliat; NF-kappa B; breast cancer
Funding
- National R&D Program for Cancer Control, Ministry of Health & Welfare, Republic of Korea [0820050]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2017R1D1A3B05030971]
- National Research Foundation of Korea [2017R1D1A3B05030971] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Vitis coignetiaePulliat (Meoru in Korea) has been used in Korean folk medicine for the treatment of inflammatory diseases and cancers. Evidence suggests that NF-kappa B activation is mainly involved in cancer cell proliferation, invasion, angiogenesis, and metastasis. TNF-alpha also enhances the inflammatory process in tumor development. Recently, flavonoids from plants have been reported to have inhibitory effects on NF-kappa B activities. We investigated the effects of anthocyanins extracted from the fruits of Vitis coignetiae Pulliat (AIM, anthocyanins isolated from Meoru (AIM)) on TNF-alpha -induced NF-kappa B activities in MCF-7 human breast cancer cells and the molecules involved in AIM-induced anti-cancer effects, especially on cancer metastasis. We performed cell viability assay, gelatin zymography, invasion assay, and western blot analysis to unravel the anti-NF-kappa B activity of AIMs on MCF-7 cells. AIM suppressed the TNF-alpha effects on the NF-kappa B-regulated proteins involved in cancer cell proliferation (COX-2, C-myc), invasion, and angiogenesis (MMP-2, MMP9, ICAM-1, and VEGF). AIM also increased the expression of E-cadherin, which is one of the hallmarks of the epithelial-mesenchymal transition (EMT) process. In conclusion, this study demonstrates that the anthocyanins isolated from the fruits of Vitis coignetiae Pulliat acts as an inhibitor of TNF-alpha induced NF-kappa B activation, and subsequent downstream molecules involved in cancer proliferation, invasion, adhesion, angiogenesis, and thus have anti-metastatic activities in MCF-7 breast cancer cells.
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