Journal
MOLECULES
Volume 25, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/molecules25051235
Keywords
Brassica juncea spp; matabolites; foods; comprehensive two-dimensional liquid chromatography; mass spectrometry; multi segmented-in-fraction gradient
Funding
- Italian Ministry of Research under the Research Project of National Interest 2015 (PRIN 2015) - Italian Ministry of University and Scientific Research [2015FFY97L]
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Plant-based foods are characterized by significant amounts of bioactive molecules with desirable health benefits beyond basic nutrition. The Brassicaceae (Cruciferae) family consists of 350 genera; among them, Brassica is the most important one, which includes some crops and species of great worldwide economic importance. In this work, the metabolite content of three different cultivars of Brassica juncea, namely ISCI Top, Broad-leaf, and ISCI 99, was determined using comprehensive two-dimensional liquid chromatography coupled with a photodiode array and mass spectrometry detection. The analyses were carried out under reversed-phase conditions in both dimensions, using a combination of a 250-mm microbore cyano column and a 50-mm RP-Amide column in the first and second dimension (D-2), respectively. A multi (three-step) segmented-in-fraction gradient for the D-2 separation was advantageously investigated here for the first time, leading to the identification of 37 metabolites. In terms of resolving power, orthogonality values ranged from 62% to 69%, whereas the corrected peak capacity values were the highest for B. juncea ISCI Top (639), followed by B. juncea Broad-leaf (502). Regarding quantification, B. juncea cv. Broad-leaf presented the highest flavonoid content (1962.61 mg/kg) followed by B. juncea cv. ISCI Top (1002.03 mg/kg) and B. juncea cv. ISCI 99 (211.37 mg/kg).
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