Journal
MOLECULAR CELL
Volume 79, Issue 1, Pages 99-+Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2020.04.026
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Funding
- MIRA from the National Institutes of Health [R35GM118026]
- Cancer Research UK Programme Foundation [CR-UK C47547/A21536]
- Wellcome Trust [200818/Z/16/Z]
- Max Planck Society
- European Research Council (ERC) [669686]
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Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin I and II and unveil the architecture of the human condensin II complex, revealing two putative DNA-entrapment sites. Using single-molecule imaging, we demonstrate that both condensin I and II exhibit ATP-dependent motor activity and promote extensive and reversible compaction of double-stranded DNA. Nucleosomes are incorporated into DNA loops during compaction without being displaced from the DNA, indicating that condensin complexes can readily act upon nucleosome-bound DNA molecules. These observations shed light on critical processes involved in genome organization in human cells.
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