Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 505, Issue -, Pages -Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2020.110729
Keywords
Pancreatic islets; Adipose-derived stem cells; Co-culture; Cytokines; Gene expression
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Funding
- Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS) [FAPERGS/CNPq 12/2014-PRONEX]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Fundo de Incentivo a Pesquisa e Eventos (FIPE) at the Hospital de Clinicas de Porto Alegre
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
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The aim of this study was to investigate whether co-culture of human islets with adipose-derived stem cells (ASCs) can improve islet quality and to evaluate which factors play a role in the protective effect of ASCs against islet dysfunction. Islets and ASCs were cultured in three experimental groups for 24 h, 48 h, and 72 h: 1) indirect co-culture of islets with ASC monolayer (Islets/ASCs); 2) islets alone; and 3) ASCs alone. Co-culture with ASCs improved islet viability and function in all culture time-points analyzed. VEGFA, HGF, IL6, IL8, IL10, CCL2, IL1B, and TNF protein levels were increased in supernatants of islet/ASC group compared to islets alone, mainly after 24 h. Moreover, VEGFA, IL6, CCL2, HIF1A, XIAP, CHOP, and NFKBIA genes were differentially expressed in islets from the co-culture condition compared to islets alone. In conclusion, co-culture of islets with ASCs promotes improvements in islet quality.
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