Journal
MICROCHIMICA ACTA
Volume 187, Issue 6, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-020-04348-x
Keywords
Aptamer; Electrospun nanofiber membrane; Antibiotic residue; Signal probes; Colorimetric assay
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Funding
- National Natural Science Foundation of China [51603034]
- Fundamental Research Funds for the Central Universities [2232017D-03]
- Seed Project of Key Laboratory of Textile Science and Technology of Ministry of Education [KLTST201622]
- 111 Project Biomedical Textile Materials Science and Technology [B07024]
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The preparation of portable colorimetric biosensor strips is described by combining aptamer-immobilized electrospun nanofiber membranes (A-NFMs) with signal probes (DNA-conjugated gold nanoparticles (AuNPs)) for determination of kanamycin (KMC) as a model analyte. The A-NFMs were decorated with complementary single-stranded DNA (cDNA) of KMC aptamer-conjugated AuNPs (cDNA@Au) to get the colorimetric biosensor strips. The constructed biosensor strips showed a significant absorbance decreasing band at 510 nm which induce a visual color change from pink to white when exposed to KMC, with a low detection limit of 2.5 nM (at S/N = 3). The effect is due to disassembling of cDNA@Au from NFMs in the presence of KMC because the aptamer has a higher affinity to KMC than its complementary DNA, which resulted in replacing cDNA@Au with KMC. Satisfactory performance was observed in real sample (drinking water and milk) analysis with a recovery of 98.9-102.2%. The constructed colorimetric biosensor test strips hold great application promise for food safety control.
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