Journal
MICROCHIMICA ACTA
Volume 187, Issue 5, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-020-04264-0
Keywords
Ratiometric strategy; Electrochemical deposition; Ionic liquid; Energy transfer; Enzyme-substrate interaction; Enzyme catalytic activity
Categories
Funding
- National Natural Science Foundation of China [21864006, 21763005, 21563006]
- Natural Science Foundation of Guangxi Province [2017GXNSFDA198034, 2017GXNSFFA198005]
- Guangxi Scientific and Technological Development Projects [AD17195081]
- Thousands of Young Teachers Training Program of Guangxi Province [guijiaoren[2018] 18]
- High-Level-Innovation Team [guijiaoren[2017]38]
- Outstanding Scholar Project of Guangxi Higher Education Institutes
- BAGUI Scholar Program of Guangxi Province of China
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A ratiometric fluorescent assay is fabricated for the evaluation of alkaline phosphatase (ALP) activity. This assay is composed of ionic liquid-functionalized carbon dots (IL-CDs) with blue fluorescence signal at 470 nm and 2,3-diaminophenazine (DAP) with yellow fluorescence signal at 570 nm. IL-CDs were synthesized via electrochemical method by using ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate) and ultrapure water as precursors. DAP is produced by the oxidation reaction between o-phenylenediamine and H2O2 under the catalysis of horseradish peroxidase. H2O2 is reduced by ascorbic acid which is the hydrolysis product of ascorbic acid 2-phosphate under the catalysis of ALP, finally reducing the amount of DAP. The activity of ALP is evaluated through the ratiometric fluorescent signal between IL-CDs and DAP via Forster resonance energy transfer. Under optimal experimental conditions, this ratiometric fluorescent assay has a response that covers the 0.04 to 3.2 U L-1 (12 to 960 pM) ALP activity. This assay possesses ultralow detection limit of 0.012 U L-1 (3.6 pM) for ALP and high selectivity for ALP among several enzymes. The method was used to measure ALP activity in human serum samples with satisfying results. Schematic presentation of IL-CDs-based ratiometric fluorescent assay for ALP activity evaluation via FRET strategy between IL-CDs and DAP. This ratiometric fluorescent assay possessed low detection limit of ALP activity (0.012 U L-1) and high selectivity among several enzymes.
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