Journal
MICROBIOLOGICAL RESEARCH
Volume 234, Issue -, Pages -Publisher
ELSEVIER GMBH
DOI: 10.1016/j.micres.2020.126426
Keywords
L-rhamnose catabolic pathway; RhaR; Inducer; Pectinolytic enzymes; Gene regulation
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Funding
- NWO [ALWOP.233]
- Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231]
- Academy of Finland [308284]
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In fungi, L-rhamnose (Rha) is converted via four enzymatic steps into pyruvate and L-lactaldehyde, which enter central carbon metabolism. In Aspergillus niger, only the genes involved in the first three steps of the Rha catabolic pathway have been identified and characterized, and the inducer of the pathway regulator RhaR remained unknown. In this study, we identified the gene (lkaA) involved in the conversion of L-2-keto-3-deoxyrhamnonate (L-KDR) into pyruvate and L-lactaldehyde, which is the last step of the Rha pathway. Deletion of lkaA resulted in impaired growth on L-rhamnose, and potentially in accumulation of L-KDR. Contrary to Delta lraA, Delta lrlA and Delta lrdA, the expression of the Rha-responsive genes that are under control of RhaR, were at the same levels in Delta lkaA and the reference strain, indicating the role of L-KDR as the inducer of the Rha pathway regulator.
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