Journal
MARINE POLLUTION BULLETIN
Volume 154, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.marpolbul.2020.111057
Keywords
Antimicrobial resistance; Beta-lactamases; Vibrios; Shewanellae; Aquaculture; Public health
Funding
- Ministry of Education, University and Research
- Project CARIVERONA 2017 entitled Diffusione della resistenza agli antibiotici carbapenemici: ricerca dei serbatoi ambientali [B31G17000640005]
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The aquatic environment can represent a reservoir of antimicrobial resistance genes. In the present study, phenotypical, biochemical and molecular techniques were used to screen a collection of marine strains isolated in Italian aquaculture farms to investigate their beta-lactam resistance profiles. The genome of 12 carbapenemase and/or beta-lactamase producing strains was sequenced and a phylogenetic analysis of the beta-lactamases found in their chromosomes was performed. Gene annotation and prediction revealed the presence of bla(AmpC) and bla(OXA-55-like) in all the Shewanella algae isolates whereas in Vibrio anguillarum and Vibrio parahaemolyticus strains, bla(AmpC) and bla(CARB-19) were found, respectively. Multiple alignments of OXA-55-like and AmpC protein sequences showed different point mutations. Finally, comparisons between enzyme phylogeny and strain clusterization based on sampling sites and dates indicate the diffusion of specific Multi Drug Resistant (MDR) Shewanella algae clones along the Italian Adriatic coast.
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