Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 123, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2020.109027
Keywords
Purine; Diazotization reaction; Uric acid; SERS; Fish meat
Categories
Funding
- Zhejiang Province Public Welfare Technology Application Research Project [LGN19C200010, LGN20C200013]
- Ningbo Public Welfare Technology Application Research Project [2019C10028, 2019C10032]
- National Key R&D Program of China [2018YFD0901105]
- Science and Technology Program of Zhejiang Food and Drug Administration [SP2018016]
- K.C. Wong Magna Fund of Ningbo University
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A new analytical method was developed to determine total purine content in fish meat. The analysis principle is that total purine extracted from fish meat are turned into uric acid by diazotization reaction and xanthine oxidase reaction. Then, the content of uric acid is measured by surface-enhanced Raman spectroscopy (SERS). The results showed that the optimal conditions for the diazotization reaction were at 60 degrees C for 40 min, using 0.16 M HCl, and 3.00 g/L NaNO2. The optimal xanthine oxidase reaction conditions were using 100 U/L xanthine oxidase, conducting at 30 degrees C and pH 7.0 for 40 min. The calibration curve established at the characteristic Raman peak of 631 cm(-1) had a good linear relationship (R-2 = 0.9864). The recovery rate was between 101.7% and 105.0%. Therefore, the total purine content in the fish meat can be quantitatively determined with this new method.
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