Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 142, Issue 15, Pages 6930-6934Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.0c01722
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Funding
- National Institute of General Medical Sciences [1R35GM128747]
- National Science Foundation [1825724]
- Div Of Civil, Mechanical, & Manufact Inn
- Directorate For Engineering [1825724] Funding Source: National Science Foundation
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Integrin-transmitted cellular forces have rich spatial dynamics and are vital to many cellular functions. To advance the sensitivity and spatial resolution of cellular force imaging, we developed a force-activatable emitter reporting single-molecular tension events and the associated cellular force nanoscopy (CFN). Immobilized on a surface, the emitters are initially dark (>99.8% quenched), providing a low fluorescence background despite the high coating density (>2000/mu m(2)) required for sampling cellular force properly. The emitters fluoresce brightly once switched on by integrin tensions and can be switched off by photobleaching, enabling continuous real-time imaging of integrin molecular tensions in live cells. With multiple cycles of molecular tension imaging and localization, CFN reproduces cellular force images with 50 nm resolution. Applied to both migratory cells and stationary cells, CFN revealed ultranarrow distribution of integrin tensions at the cell leading edge, and showed that force distribution in focal adhesions (FAs) is off-centered and FA size-dependent.
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