Journal
JOURNAL OF PHARMACY AND PHARMACOLOGY
Volume 72, Issue 8, Pages 1101-1109Publisher
OXFORD UNIV PRESS
DOI: 10.1111/jphp.13280
Keywords
diabetic nephropathy; H19; metformin; miR-143-3p; TGF-beta 1
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Funding
- Local Science and Technology Development Project Guide by The Central Government of China [2017070802D147]
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Objectives Metformin (MET) has protective effect on diabetic nephropathy (DN). This study aims to demystify the mechanism of MET function in DN. Methods Mouse glomerular membrane epithelial cell line SV40-MES-13 was treated with normal or high glucose combined with or without MET. The relationships among H19, miR-143-3p and TGF-beta 1 were evaluated by luciferase reporter assay. MTT assay was performed to detect cell proliferation. The levels of inflammatory factors were investigated by enzyme-linked immunosorbent assay. Quantitative real-time PCR and western blot were performed to examine gene and protein expression. Results H19 was up-regulated in the SV40-MES-13 cells after treated with high glucose, which was effectively repressed by MET treatment. MET promoted extracellular matrix accumulation, inflammation and proliferation in the SV40-MES-13 cells after treated with high glucose. These influences conferred by MET were abolished by H19 overexpression. H19 regulated TGF-beta 1 expression by sponging miR-143-3p. Furthermore, MET inhibited extracellular matrix accumulation, inflammation and proliferation by regulating H19/miR-143-3p/TGF-beta 1 axis. Conclusions Our studies demonstrated that the protective effect of MET on DN was attributed to the inhibition of proliferation, inflammation and ECM accumulation in mesangial cells via H19/miR-143-3p/TGF-beta 1 axis, which suggested that the H19/miR-143-3p/TGF-beta 1 axis could be a valuable target for DN therapies.
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