Journal
JOURNAL OF NEUROSCIENCE
Volume 40, Issue 17, Pages 3491-3501Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2880-19.2020
Keywords
Alzheimer's disease; E/I balance; PET; synapse; tau
Categories
Funding
- AMED [JP19dm0207072, JP19dk0207049, JP18dm0107062, 17dm0107066h]
- JST CREST [JPMJCR1652]
- Center of Innovation Program [JPMJCE1305]
- JSPS KAKENHI [6K21636, 18H04752, 18K07777]
- Grants-in-Aid for Scientific Research [18H04752, 18K07777] Funding Source: KAKEN
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Synaptic dysfunction provoking dysregulated cortical neural circuits is currently hypothesized as a key pathophysiological process underlying clinical manifestations in Alzheimer's disease and related neurodegenerative tauopathies. Here, we conducted PET along with postmortem assays to investigate time course changes of excitatory and inhibitory synaptic constituents in an rTg4510 mouse model of tauopathy, which develops tau pathologies leading to noticeable brain atrophy at 5-6 months of age. Both male and female mice were analyzed in this study. We observed that radiosignals derived from [C-11]flumazenil, a tracer for benzodiazepine receptor, in rTg4510 mice were significantly lower than the levels in nontransgenic littermates at 2-3 months of age. In contrast, retentions of (E)-[C-11]ABP688, a tracer for mGluR5, were unaltered relative to controls at 2 months of age but then gradually declined with aging in parallel with progressive brain atrophy. Biochemical and immunohistochemical assessment of postmortem brain tissues demonstrated that inhibitory, but not excitatory, synaptic constituents selectively diminished without overt loss of somas of GABAergic interneurons in the neocortex and hippocampus of rTg4510 mice at 2 months of age, which was concurrent with enhanced immunoreactivity of cFos, a well-characterized immediate early gene, suggesting that impaired inhibitory neurotransmission may cause hyperexcitability of cortical circuits. Our findings indicate that tau-induced disruption of the inhibitory synapse may be a critical trigger of progressive neurodegeneration, resulting in massive neuronal loss, and PET assessments of inhibitory versus excitatory synapses potentially offer in vivo indices for hyperexcitability and excitotoxicity early in the etiologic pathway of neurodegenerative tauopathies.
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