Journal
CHEMICAL COMMUNICATIONS
Volume 52, Issue 22, Pages 4219-4222Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc08907g
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Funding
- Singapore Millennium Foundation
- Singapore Ministry of Education Academic Research Fund
- National University of Singapore
- Ministry of Education
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We established four-point guidelines for the sequence design of hairpin monomers in hybridization chain reaction (HCR). This enabled greater flexibility to customize specific hairpin sequences for use with the readout platform of interest. Using shorter hairpin stem length, a one-pot signal amplification system was demonstrated by incorporating distance-sensitive Forster resonance energy transfer (FRET) readout.
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