Journal
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
Volume 20, Issue 6, Pages 3348-3355Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2020.17402
Keywords
Alkaline Phosphatase; ALP; Fluorescent Probe; CyP
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Funding
- National Natural Science Foundation of China [U1809214]
- Science and Technology Plan Project of Zhou Shan City [2018C21012, 2016C41006]
- Fundamental Research Funds for the Provincial Universities in Zhejiang Province [2019J00033]
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In marine environment, alkaline phosphatase (ALP) is a nonspecific phosphatase with metal ions as its active site. Metal ions have different effects on ALP activity. Therefore, a probe that specifically detects ALP needs to be developed. In this paper, to eliminate the interference of acid phosphatase, we designed and synthesized a highly selective fluorescent probe CyP based on pH to detect ALP activity. The response mechanism of detecting ALP was explained. The photophysical properties, enzyme kinetics, stability, selectivity, and potential quantitative ability of the probe under different pH values were investigated. The effects of metal ions on the ALP activity of marine Chlorella vulgaris and Escherichia coil were also analyzed. Excessive metal ions such as Zn2+, Cu2+, Hg2+, Pb2+, and Cd2+ inhibit while Mn2+, Co2+, and alkaline-earth metal ions promote the ALP activity of Chlorella and E. coli.
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