Journal
CHEMICAL COMMUNICATIONS
Volume 52, Issue 2, Pages 406-409Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc06754e
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Funding
- National Natural Science Foundation of China [21105095, 211900040]
- State Key Instrument Developing Special Project of Ministry of Science and Technology of China [2012YQ170003]
- Instrument Developing Project of the Chinese Academy of Sciences [YZ201203]
- Natural Science Foundation of Jilin Province, China [20130101117JC]
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With CCRF-CEM as the model cell, a highly sensitive and selective cytosensor was developed by taking advantage of polydopamine nanospheres for the first time. The strategies of aptamer/membrane protein recognition and Exonuclease III assisted cycle amplification were used for improving selectivity and sensitivity. The detection of limit reached was as low as 15 cells per mL.
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