Journal
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
Volume 62, Issue 11, Pages 1688-1702Publisher
WILEY
DOI: 10.1111/jipb.12943
Keywords
anthocyanin; rosmarinic acid; salvianolic acid B; Salvia miltiorrhiza; SmMYB2
Categories
Funding
- National Natural Science Fund of China [81522049, 31571735, 31270007, 30900110]
- Dawn Program of Shanghai Education Commission [16SG38]
- Shanghai Science and Technology Committee Project [17JC1404300, 15430502700]
- Zhejiang Provincial Wanren Program for Leading Talents of Science and Technology Innovation [2018R52050]
- Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents [2018-62-3]
- New Century Talent Project [NECT-13-0902]
- Opening Project of Zhejiang Provincial Preponderant and Characteristic Subject of Key University (Traditional Chinese Pharmacology), Zhejiang Chinese Medical University [ZYAOX2018019]
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MYB transcription factors play vital roles in plant growth and metabolism. The phytohormone methyl jasmonate (MeJA) promotes phenolic acid accumulation in the medicinal herb Salvia miltiorrhiza, but the regulatory mechanism is poorly understood. Here, we identified the MeJA-responsive R2R3-MYB transcription factor gene SmMYB2 from a transcriptome library produced from MeJA-treated S. miltiorrhiza hairy roots. SmMYB2 expression was tightly correlated with the expression of key salvianolic acid biosynthetic genes including CYP98A14. SmMYB2 was highly expressed in the periderm of S. miltiorrhiza and SmMYB2 localized to the nucleus. Overexpressing SmMYB2 in S. miltiorrhiza hairy roots significantly increased the levels of salvianolic acids (including rosmarinic acid and salvianolic acid B) by upregulating salvianolic acid biosynthetic genes such as CYP98A14. SmMYB2 binds to the MYB-binding motifs in the promoter of CYP98A14, as confirmed by a dual-luciferase assay and electrophoretic mobility shift assays. Anthocyanin contents were significantly higher in SmMYB2-overexpressing hairy root lines than the control, primarily due to the increased expression of CHI, DFR, and ANS. These findings reveal the novel regulatory role of SmMYB2 in MeJA-mediated phenolic acid biosynthesis, providing a useful target gene for metabolic engineering and shedding light on the salvianolic acid regulatory network.
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