Journal
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
Volume 62, Issue 10, Pages 1500-1517Publisher
WILEY
DOI: 10.1111/jipb.12931
Keywords
Arabidopsis thaliana; Brassinosteroids signaling; BES1; Auxin signaling; PIN7; SHY2; Root meristem; Transcriptional regulation
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Funding
- National Natural Science Foundation of China [31670235, 31970263, 31972258]
- Development Project of Transgenic Crops of China [2016ZX08009-003-002]
- Fundamental Research Funds for the Central Universities [SCU2019D013]
- Applied basic project of Sichuan Science and Technology Department [2018JY0192]
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In multicellular organisms, the balance between cell division and differentiation determines organ size, and represents a central unknown in developmental biology. In Arabidopsis roots, this balance is mediated between cytokinin and auxin through a regulatory circuit converging on the IAA3/SHORT HYPOCOTYL 2 (SHY2) gene. Here, we show that crosstalk between brassinosteroids (BRs) and auxin occurs in the vascular transition zone to promote root meristem development. We found that BR increases root meristem size by up-regulating expression of the PINFORMED 7 (PIN7) gene and down-regulating expression of the SHY2 gene. In addition, BES1 could directly bind to the promoter regions of both PIN7 and SHY2, indicating that PIN7 and SHY2 mediate the BR-induced growth of the root meristem by serving as direct targets of BES1. Moreover, the PIN7 overexpression and loss-of-function SHY2 mutant were sensitive to the effects of BR and could partially suppress the short-root phenotypes associated with deficient BR signaling. Interestingly, BRs could inhibit the accumulation of SHY2 protein in response to cytokinin. Taken together, these findings suggest that a complex equilibrium model exists in which regulatory interactions among BRs, auxin, and cytokinin regulate optimal root growth.
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