Journal
JOURNAL OF INFECTIOUS DISEASES
Volume 222, Issue 2, Pages 189-193Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiaa243
Keywords
chemiluminescence immunoassay; COVID-19; SARS-CoV-2; serological test
Categories
Funding
- National Science and Technology Major Project [2017ZX10202203, 2018ZX10732202]
- Chongqing Natural Science Foundation [cstc2020jscx-fyzx0053, cstc2020jscx-fyzx0050, cstc2018jscx-msybX0032]
- Natural Science Foundation of Chongqing Yuzhong [20190145]
Ask authors/readers for more resources
Background. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel beta-coronavirus, causes severe pneumonia and has spread throughout the globe rapidly. The disease associated with SARS-CoV-2 infection is named coronavirus disease 2019 (COVID-19). To date, real-time reverse-transcription polymerase chain reaction (RT-PCR) is the only test able to confirm this infection. However, the accuracy of RT-PCR depends on several factors; variations in these factors might significantly lower the sensitivity of detection. Methods. In this study, we developed a peptide-based luminescent immunoassay that detected immunoglobulin (Ig)G and IgM. The assay cutoff value was determined by evaluating the sera from healthy and infected patients for pathogens other than SA RS-CoV-2. Results. To evaluate assay performance, we detected IgG and IgM in the sera from confirmed patients. The positive rate of IgG and IgM was 71.4% and 57.2%, respectively. Conclusions. Therefore, combining our immunoassay with real-time RT-PCR might enhance the diagnostic accuracy of COVID-19.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available