Journal
JOURNAL OF IMMUNOLOGY
Volume 205, Issue 1, Pages 251-260Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1900801
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Funding
- National Institutes of Health [GM-29507, GM-61656]
- Stobbe Endowment of the Department of Pathology at University of Michigan Medical School
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Over the first days of polymicrobial sepsis, there is robust activation of the innate immune system, causing the appearance of proinflammatory cytokines and chemokines, along with the appearance of extracellular histones, which are highly proinflammatory and prothrombotic. In the current study, we studied different innate immune responses in mice with knockout (KO) of complement protein 6 (C6). Polymorphonuclear neutrophils (PMNs) from these KO mice had defective innate immune responses, including defective expression of surface adhesion molecules, generation of superoxide anion, and appearance of reactive oxygen species and histone release after activation of PMNs, along with defective phagocytosis. In addition, in C6(-/-) mice, the NLRP3 inflammasome was defective both in PMNs and in macrophages. When these KO mice were subjected to polymicrobial sepsis, their survival was improved, associated with reduced levels in the plasma of proinflammatory cytokines and chemokines and lower levels of histones in plasma. In addition, sepsis-induced cardiac dysfunction was attenuated in these KO mice. In a model of acute lung injury induced by LPS, C6(-/-) mice showed reduced PMN buildup and less lung epithelial/endothelial cell dysfunction (edema and hemorrhage). These data indicate that C6(-/-) mice have reduced innate immune responses that result in less organ injury and improved survival after polymicrobial sepsis.
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