Journal
CHEMICAL COMMUNICATIONS
Volume 52, Issue 87, Pages 12889-12892Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6cc05796a
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Funding
- Japan Society for the Promotion of Science [26810086, 15H01062, 15K13738, 26288075]
- Biomaterials Analysis Division, Technical Department, Tokyo Institute of Technology
- Grants-in-Aid for Scientific Research [26810086, 15H01062, 26288075, 15K13738, 16K05840] Funding Source: KAKEN
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Enzymatic synthesis and the reverse transcription of RNAs containing 2'-O-carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the synthesis of 2'-O-carbamoyl uridine triphosphate (UcmTP). UcmTP was incorporated correctly into long RNAs, and its fidelity during reverse transcription using SuperScript III was sufficient for RNA aptamer selection.
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