Journal
CHEMICAL COMMUNICATIONS
Volume 52, Issue 6, Pages 1218-1221Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc08797j
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [21325523, 21527811]
- Award for the Hundred Talent Program of Nanyue of Guangdong Province
- Award for Pengcheng Distinguished Scholars of Shenzhen City
- Award for Team Leader Program of Taishan Scholars of Shandong Province, China
Ask authors/readers for more resources
We develop a sensitive and selective method for the multiplex detection of histone-modifying enzymes (HMEs) through the integration of antibody-based fluorescence labeling with total internal reflection fluorescence (TIRF)-based single-molecule detection. This method exhibits excellent specificity and high sensitivity with a detection limit of 21 pM for histone acetyltransferase GcN5 and 12 pM for histone methyltransferase G9a, and it can be applied for the screening of HME inhibitors as well.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
