Journal
CHEMICAL COMMUNICATIONS
Volume 52, Issue 43, Pages 6997-7000Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6cc02764d
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Funding
- University of Wisconsin-Madison
- National Institutes of Health [NIBIB/NCI 1R01CA169365, P30CA014520, T32CA009206]
- American Cancer Society [125246-RSG-13-099-01-CCE]
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A small luciferase protein (Nluc) was conjugated to QDs as a bioluminescence resonance energy transfer (BRET) pair. The conjugate showed 76% BRET efficiency and lymph node mapping was successfully performed. The cRGD peptide was conjugated to QD-Nluc for tumor targeting. The self-illuminating QD-Nluc showed excellent energy transfer in a living system and offered an optimal tumor-to-background ratio (>85).
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