Journal
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
Volume 57, Issue 8, Pages 2877-2883Publisher
SPRINGER INDIA
DOI: 10.1007/s13197-020-04319-3
Keywords
Donkey-hide gelatines (DHG); Loop-mediated isothermal amplification (LAMP); Authentication
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Donkey-hide gelatine (DHG) is a well-known, animal-derived traditional Chinese medicine material calledColla corii asini(known in Chinese as E'jiao). Because DHG is claimed to have properties that are beneficial to health, its consumption has increased, but its production has decreased. Thus, the incidence of DHG adulteration has become increasingly serious. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for the authentication of DHG. Identification of donkey DNA from DHG was performed specifically and rapidly within one hour by LAMP primers. Moreover, the sensitivity of LAMP in authenticating DHG was 10(-3) ng, which revealed a 10(5)-fold higher sensitivity than that of conventional PCR. The relative detection limit was 0.1% DHG in the adulterants, including gelatines of horse, cow, pork, goat, sheep or chicken origins. When genomic DNAs extracted from heat-treated DHG samples, including boiling or autoclaving for 40 min, were used as templates, DHG detection by LAMP was unchanged and reproducible. In conclusion, the LAMP assay established herein could potentially be applied for the authentication of DHG and DHG-related products in herbal or food markets.
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