Journal
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
Volume 87, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jfca.2019.103393
Keywords
High-affinity liquid chromatography (HPLC); HILIC affinity column; Sialic acid; Food analysis; Food composition; Sample pretreatment optimization
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Funding
- Jilin Institute for Food Control [spaq-2018-044]
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We have established a novel hydrophilic chromatography (HILIC)-high performance liquid chromatography (HPLC) method to assess sialic add content in food products. Single-factor and response surface methodologies (RSM) were used to systematically optimize the hydrolysis conditions of the food samples to extract the maximum amount of sialic acid. Chromatographic conditions were also adjusted. In foods containing sialic acid, we observed a strong linear relationship between sialic acid and peak area, ranging from 5 to 100 mu g/mL (R-2 = 0.9998). The lowest detectable sialic acid concentration (RSN = 3) was 0.2 mu g mL(-1), and the method detection limit was 0.02mg kg(-1). Sample recovery ranged from 95.85% to 99.78%, with an RSD of 1.46% (n = 6). Thus, the described method can be applied to the study of sialic add content in foods.
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