Journal
JOURNAL OF FISH BIOLOGY
Volume 97, Issue 2, Pages 374-382Publisher
WILEY
DOI: 10.1111/jfb.14366
Keywords
11-ketotestosterone; beluga; dmrt1; foxl2; gonadotropin receptors; ovary; previtellogenesis
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Funding
- Caspian Sea Basin Research Center, University of Guilan [1853959]
- Spanish Ministry of Economy and Competiveness
- EU-FEDER/ERDF project ATREoVO [AGL2015-63936-R]
- European funding (ERDF)
- European funding (ESF)
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Thein vivoeffect of 11-ketotestosterone (11KT) on transcript levels of the gonadotropin receptors (fshrandlhr) and sex differentiation-related genes (dmrt1andfoxl2) was examined in the ovaries of immature female beluga. For this purpose, six fish were treated with implants containing 2.5 mg 11KT and a placebo group of six females of the same age and gametogenic stage were given a blank implant. The implants were intraperitoneally inserted into 4-year-old females at the previtellogenic stage (mean body weight 5580 +/- 165 g) and maintained under culture conditions for 8 weeks. Ovary samples for gene expression analysis oflhr,fshr,dmrt1andfoxl2were collected by biopsy at 3 and 8 weeks post implantation. Diameters of oocytes increased in response to 11KT treatment, both at 3 and at 8 weeks post implantation, but no obvious changes were evident in cytology. Three weeks of 11KT treatment did not affect target gene expression, but a tendency for a time-dependent decrease oflhranddmrt1mRNA levels was observed in both treatment and placebo groups. By 8 weeks of treatment, however, 11KT implants provoked the upregulation offshrandfoxl2transcript levels. Furthermore,lhranddmrt1transcript abundances recovered by 8 weeks of exposure in both blank- and 11KT-implanted beluga. These results suggest that 11KT, either directly or indirectly, may affect gametogenesis and regulate some key components of the reproductive axis in female beluga.
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