4.7 Article

Fluorogenic bidirectional displacement probebased real-time isothermal DNA amplification and specific visual detection of products

Journal

CHEMICAL COMMUNICATIONS
Volume 52, Issue 76, Pages 11438-11441

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c6cc05158h

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Funding

  1. National Natural Science Foundation of China [31270907]
  2. National Key Foundation for Exploring Scientific Instrument [2013YQ470781]
  3. Autonomous Research Project of the State Key Laboratory of Industrial Control Technology, China

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A unique fluorogenic nucleic acid probe has been devised, called the fluorogenic bidirectional displacement probe, which can serve as both the primer and the signal indicator of amplification products for the development of the real-time isothermal DNA amplification and its visual detection of products with high sensitivity and specificity.

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