Journal
JOURNAL OF CONTROLLED RELEASE
Volume 320, Issue -, Pages 63-72Publisher
ELSEVIER
DOI: 10.1016/j.jconrel.2020.01.005
Keywords
Immune checkpoint inhibitor; p-Hydroxybenzoic acid; Conjugate; Brain-targeting; Glioblastoma
Funding
- National Natural Science Foundation of China [81690263]
- Shanghai Education Commission Major Project [2017-01-07-00-07-E00052]
- National Basic Research Program of China (973 Program) [2013CB932500]
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In glioblastoma with typical immunosuppressive characteristics, immune checkpoint inhibitors treatment showed unsatisfactory clinical effects, attributable to the exclusion of antibodies by blood-brain barrier (BBB) to a large extent. Herein, a conjugate of anti-programmed death ligand 1 antibody (alpha PDL1) and the targeting moiety p-hydroxybenzoic acid (pHA) was designed to realize crossing BBB of antibody based on the dopamine receptor mediated transcytosis. Conjugation with pHA did not influence the binding affinity of alpha PDL1 with PD-L1 protein, thus maintaining the capability of PD pathway blockade. Importantly, pHA-alpha PDL1 crossed BBB, demonstrated by the increased distribution in both the brain and the glioma after intravenous administration of pHA-alpha PDL1. Compared with the unmodified alpha PDL1, pHA-alpha PDL1 prolonged the survival time and suppressed tumor growth more effectively in an orthotopic glioblastoma model by activating glioma-infiltrating T cells. Our results suggested the potential of the antibody-pHA conjugate to improve efficacy for cerebral diseases by providing a potential platform for macromolecules to play therapeutics role in the brain.
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