4.7 Article

Plug-and-playable fluorescent cell imaging modular toolkits using the bacterial superglue, SpyTag/SpyCatcher

Journal

CHEMICAL COMMUNICATIONS
Volume 52, Issue 97, Pages 14051-14054

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c6cc07363h

Keywords

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Funding

  1. National Research Foundation of Korea [NRF-2010-0028684, NRF-2016R1D1A1B03932580]
  2. UNIST [1.160060.01]
  3. National Research Foundation of Korea [22A20130012280, 2016R1D1A1B03932580, 2016H1A2A1907554] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

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Simple plug-and-playable fluorescent cell imaging modular toolkits are established using the bacterial superglue SpyTag/SpyCatcher protein ligation system. A variety of affibody-fluorescent protein conjugates (AFPCs) are post-translationally generated via the isopeptide bond formation, and each AFPC effectively recognizes and binds to its targeting cells, visualizing them with selective colors on demand.

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