Journal
CHEMICAL COMMUNICATIONS
Volume 52, Issue 23, Pages 4365-4368Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6cc00383d
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We have engineered a hairpin ribozyme that in a two-step reaction supports RNA recombination by catalysing the cleavage of two non-functional RNA precursors (first step) followed by the recombination of two of the cleavage fragments into a functional RNA (second step). The recombination product (yield: 76%) is a fully functional hammerhead ribozyme.
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