Journal
CHEMICAL COMMUNICATIONS
Volume 52, Issue 21, Pages 4041-4044Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc08816j
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- A-Step from Japan Science and Technology Agency [AS231Z04438F]
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We developed a light-up fluorophore-DNA aptamer pair for label-free aptamer sensors that fluoresce upon binding to the analyte. A 42mer DNA aptamer binding to the environment-sensitive fluorophore, dapoxyl, which increased the fluorescence by more than 700-fold upon binding, was successfully used to construct aptamer sensors by fusion with analyte-binding DNA aptamers.
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