Journal
JOURNAL OF CELLULAR PHYSIOLOGY
Volume 235, Issue 10, Pages 7251-7260Publisher
WILEY
DOI: 10.1002/jcp.29624
Keywords
cancer stem; initiating cells; fibroblasts; Fucci; invasion; podoplanin; squamous cell carcinoma
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Funding
- Japan Society for the Promotion of Science [16H05311, 24659185]
- National Cancer Center Research and Development Fund [23-A-12]
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To clear whether podoplanin-positive cancer stem cells in squamous cell carcinoma have higher invasion activity during a fibroblasts-dependent invasion. A collagen gel invasion assay was performed using fluorescent ubiquitination-based cell cycle indicator-labeled A431 cells. The total number and number of invading cells in S/G2/M phase were counted using time-lapse imaging cocultured with fibroblasts. There was no significant difference between the number of invading podoplanin-positive and negative A431 cells when fibroblasts did not exist. On the contrary, the number of invading podoplanin-positive cells was significantly higher when fibroblasts existed. The frequency of cells in S/G2/M phase among invasion was no difference. Knockdown of podoplanin decreased the number of invaded A431 cells significantly when fibroblasts existed. Podoplanin-positive A431 cells display higher invasion activity when fibroblasts exist, suggesting that some biological functions of cancer stem cells might become evident only within the fibrous tumor microenvironment.
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