Journal
JOURNAL OF CELLULAR PHYSIOLOGY
Volume 235, Issue 11, Pages 8640-8652Publisher
WILEY
DOI: 10.1002/jcp.29708
Keywords
Activin A; BMP4; EpiSCs; mouse strain; multipotent progenitors
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Funding
- National Natural Science Foundation of China [31560335]
- Ministry of Science and Technology Project of Inner Mongolia [20130216]
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We recently reported that epiblast stem cells (EpiSCs)-like cells could be derived from preimplantation embryos (named as AFSCs). Here, we established AFSCs from pre-implantation embryos of multiple mouse strains and showed that unlike EpiSCs, the derivation efficiency of AFSCs was affected by the genetic background. We then used AFSCs lines to dissect the roles of Activin A (Act A) and basic fibroblast growth factor and reported that Act A alone was capable of maintaining self-renewal but not developmental potential in vivo. Finally, we established a novel experimental system, in which AFSCs were efficiently converted to multipotent progenitor stem cells using Act A and bone morphogenetic protein 4 (named as ABSCs). Importantly, these ABSCs contributed to neural mesodermal progenitors and lateral plate mesoderm in postimplantation chimeras. Taken together, our study established a robust experimental system for the generation of specific multipotent progenitor stem cells that was self-renewable and capable of contributing to embryonic and extra-embryonic tissues.
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