Journal
JOURNAL OF CELL SCIENCE
Volume 133, Issue 8, Pages -Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.236778
Keywords
Membrane bleb; Integrin; ECM reorganization; Cancer; Cell migration
Categories
Funding
- National Institutes of Health/National Cancer Institute Cancer Center [P30CA013696]
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The study of cancer cell invasion in 3D environments in vitro has revealed a variety of invasive modes, including amoeboid migration, characterized by primarily round cells that invade in a protease- and adhesion-independent manner. Here, we delineate a contractility-dependent migratory mode of primarily round breast cancer cells that is associated with extensive integrin-mediated extracellular matrix (ECM) reorganization that occurs at membrane blebs, with bleb necks sites of integrin clustering and integrin-dependent ECM alignment. We show that the spatiotemporal distribution of blebs and their utilization for ECM reorganization is mediated by functional beta 1 integrin receptors and other components of focal adhesions. Taken together, the work presented here characterizes a migratory mode of primarily round cancer cells in complex 3D environments and reveals a fundamentally new function for membrane blebs in cancer cell invasion.
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