Journal
JOURNAL OF CELL BIOLOGY
Volume 219, Issue 7, Pages -Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201910177
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Funding
- Swiss National Science Foundation [31003A_17303]
- Novartis Foundation for medical-biological research [19B140]
- Intramural Research Program of the National Institute of Diabetes and Digestive and Kidney Diseases
- European Union's Horizon 2020 Research and Innovation Programme under the Marie Sklodowska-Curie grant [747536]
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [ZIADK060004] Funding Source: NIH RePORTER
- Marie Curie Actions (MSCA) [747536] Funding Source: Marie Curie Actions (MSCA)
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Lipid droplets (LDs) are fat storage organelles that originate from the endoplasmic reticulum (ER). Relatively little is known about how sites of LD formation are selected and which proteins/lipids are necessary for the process. Here, we show that LDs induced by the yeast triacylglycerol (TAG)-synthases Lro1 and Dga1 are formed at discrete ER subdomains defined by seipin (Fld1), and a regulator of diacylglycerol (DAG) production, Nem1. Fld1 and Nem1 colocalize to ER-LD contact sites. We find that Fld1 and Nem1 localize to ER subdomains independently of each other and of LDs, but both are required for the subdomains to recruit the TAG-synthases and additional LD biogenesis factors: Yft2, Pex30, Pet10, and Erg6. These subdomains become enriched in DAG. We conclude that Fld1 and Nem1 are both necessary to recruit proteins to ER subdomains where LD biogenesis occurs.
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