Global transcriptome analysis of novel stress associated protein (SAP) genes expression dynamism of combined abiotic stresses inOryza sativa(L.)

Genes encoding proteins with A20/AN1 zinc-finger domains, belonging to the stress associated protein (SAP) gene family, are present in all eukaryotes and play a decisive role in plant response to diverse physiological and molecular activities particularly on biotic and abiotic stresses (AbS). In this first and foremost study, global transcriptome analysis of members of theSAPgene family was carried out in C3 model-Oryza sativa(OsSAP) aiming at the identification ofOsSAPgenes activated in response to unique or Combined AbS (CAbS). Based on the available spatio-temporal and phytohormonal RNA-Seq expression profile datasets, nineOsSAPgenes were filtered out and identified by a differential expression signature noted in various tissues as well as plant hormones. Comparative genome ideogram ofOsSAPgenes confirmed the orthologous collinearity with C4 panicoid genomes. Interactome of these genes, revealed the molecular cross-talks ofOsSAP. Thus, the computational expression signature ofOsSAPgenes led to a better understanding of gene dynamism in diverse developmental tissues/organs. Transcriptional regulation analysis of keyOsSAPgenes in response to stress (drought and salinity) suggested the novel role ofOsSAP1,OsSAP2,OsSAP5, OsSAP7,OsSAP8andOsSAP11in AbS. Altogether, the study provides deeper insights on molecular characteristics ofOsSAPgenes, which could be deployed further to decipher their precise functional roles in AbS responses. Communicated by Ramaswamy H. Sarma

Automated summary

In this study, the global transcriptome analysis of the OsSAP gene family revealed their crucial role in plant response to biotic and abiotic stresses. Computational expression signature provided insights into the dynamic characteristics of these genes in diverse developmental tissues/organs. Transcriptional regulation analysis highlighted the novel roles of key OsSAP genes in response to stress.