Journal
JOURNAL OF BIOMOLECULAR NMR
Volume 74, Issue 4-5, Pages 223-228Publisher
SPRINGER
DOI: 10.1007/s10858-020-00312-2
Keywords
Methyl TROSY; Adiabatic relaxation dispersion; Composite decoupling; Cross-correlation between DD and CSA; Methyl relaxation; Conformational dynamics
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Funding
- Intramural Research Program of the National Cancer Institute [ZIA BC 011131]
- NATIONAL CANCER INSTITUTE [ZIABC011131, ZIABC011132] Funding Source: NIH RePORTER
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Recent methyl adiabatic relaxation dispersion experiments provide examination of conformational dynamics across a very wide timescale (10(2)-10(5) s(-1)) and, particularly, provide insight into the hydrophobic core of proteins and allosteric effects associated with modulators. The experiments require efficient decoupling of H-1 and C-13 spin interactions, and some artifacts have been discovered, which are associated with the design of the proton decoupling scheme. The experimental data suggest that the original design is valid; however, pulse sequences with either no proton decoupling or proton decoupling with imperfect pulses can potentially exhibit complications in the experiments. Here, we demonstrate that pulse imperfections in the proton decoupling scheme can be dramatically alleviated by using a single composite pi pulse and provide pure single-exponential relaxation data. It allows the opportunity to access high-quality methyl adiabatic relaxation dispersion data by removing the cross-correlation between dipole-dipole interaction and chemical shift anisotropy. The resulting high-quality data is illustrated with the binding of an allosteric modulator (G2BR) to the ubiquitin conjugating enzyme Ube2g2.
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