Journal
CHEMBIOCHEM
Volume 17, Issue 14, Pages 1323-1327Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201600230
Keywords
acid sensitivity; ion channels; merocyanine; photoactivation
Funding
- National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health (NIH) [R01K019038]
- W.M. Keck Foundation
- Arnold and Mabel Beckman Foundation
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We describe the reversible photoactivation of the acid sensitive ligand-gated ion channel ASIC2a, a mammalian channel found throughout the central and peripheral nervous systems that is associated with vision and pain. We also show the activation of GLIC, an acid-sensitive prokaryotic homologue of the nicotinic acetylcholine receptor. Photoactivation was achieved by using visible light irradiation of a newly synthesized water-soluble merocyanine photoacid, 1, which was designed to remove adverse channel blocking effects of a related system. Activation of ASIC2a and GLIC occurs reversibly, in a benign manner, and only upon irradiation. Further studies using transient absorption spectroscopy showed that protonation of a colorimetric base occurred rapidly (ca. 100 ms) after excitation of 1. These results demonstrate that irradiation of 1 can induce rapid, local pH changes that can be used to investigate both biological and chemical proton transfer reactions.
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