Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 295, Issue 17, Pages 5717-5736Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.RA119.011946
Keywords
calcium; drug resistance; phospholipase C; phosphoinositide; inositol phospholipid; inositol phosphate; Rac (Rac GTPase); tyrosine?protein kinase (tyrosine kinase); B-cell receptor (BCR); B-cell signaling; Bruton's tyrosine kinase (BTK); chronic lymphocytic leukemia (CLL); ibrutinib
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Funding
- Excellence Initiative of the German Federal and State Governments Grant [GSC 279]
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Treatment of patients with chronic lymphocytic leukemia (CLL) with inhibitors of Bruton's tyrosine kinase (BTK), such as ibrutinib, is limited by primary or secondary resistance to this drug. Examinations of CLL patients with late relapses while on ibrutinib, which inhibits BTK's catalytic activity, revealed several mutations in BTK, most frequently resulting in the C481S substitution, and disclosed many mutations in PLCG2, encoding phospholipase C-?(2) (PLC?(2)). The PLC?(2) variants typically do not exhibit constitutive activity in cell-free systems, leading to the suggestion that in intact cells they are hypersensitive to Rac family small GTPases or to the upstream kinases spleen-associated tyrosine kinase (SYK) and Lck/Yes-related novel tyrosine kinase (LYN). The sensitivity of the PLC?(2) variants to BTK itself has remained unknown. Here, using genetically-modified DT40 B lymphocytes, along with various biochemical assays, including analysis of PLC?(2)-mediated inositol phosphate formation, inositol phospholipid assessments, fluorescence recovery after photobleaching (FRAP) static laser microscopy, and determination of intracellular calcium ([Ca2+](i)), we show that various CLL-specific PLC?(2) variants such as PLC?(2)S707Y are hyper-responsive to activated BTK, even in the absence of BTK's catalytic activity and independently of enhanced PLC?(2) phospholipid substrate supply. At high levels of B-cell receptor (BCR) activation, which may occur in individual CLL patients, catalytically-inactive BTK restored the ability of the BCR to mediate increases in [Ca2+](i). Because catalytically-inactive BTK is insensitive to active-site BTK inhibitors, the mechanism involving the noncatalytic BTK uncovered here may contribute to preexisting reduced sensitivity or even primary resistance of CLL to these drugs.
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