Journal
CHEMBIOCHEM
Volume 18, Issue 1, Pages 72-76Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201600328
Keywords
5-carboxycytidine; 5-formylcytidine; 5-methylcytidine; RNA; TET enzyme
Funding
- University at Albany, State University of New York
- Simons Foundation [338863]
- Cancer Prevention and Research Institute of Texas [RR140053]
- Innovation Award from American Heart Association [16IRG27250155]
- Welch Foundation [BE-1913]
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It was recently revealed that 5-methylcytosine (5mC) in mRNA, similar to its behavior in DNA, can be oxidized to produce 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC), implying the potential regulatory roles of this post-transcriptional RNA modification. In this study, we demonstrate the in vitro oxidation of 5fC to 5-carboxycytidine (5caC) by the catalytic domain of mammalian ten-eleven translocation enzyme (TET1) in different RNA contexts. We observed that this oxidation process has very low sequence dependence and can take place in single-stranded, double-stranded, or hairpin forms of RNA sequences, although the overall conversion yields are low.
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