Journal
CHEMBIOCHEM
Volume 17, Issue 3, Pages 218-223Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201500541
Keywords
biosynthesis; dehydroalanine; glutamylation; goadsporin; natural products; RiPPs
Funding
- IFO, Institute for Fermentation, Osaka
- KAKENHI [26850043, 26850044, 25108707]
- Grants-in-Aid for Scientific Research [26850044, 25108707, 26850043] Funding Source: KAKEN
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Dehydroalanines in goadsporin are proposed to be formed by GodF and GodG, which show slight homology to the N-terminal glutamylation and C-terminal elimination domains, respectively, of LanB, a classI lanthipeptide dehydratase. Although similar, separated-type LanBs are conserved among thiopeptides and indispensable for their biosynthesis and biological activities, these enzymes had not yet been characterized. Here, we identified goadsporinB, which has unmodified Ser4 and Ser14, from both godF and godG disruptants. The godG disruptant also produced goadsporinC, a glutamylated-Ser4 variant of goadsporinB. These results suggested that dehydroalanines are formed by glutamylation and glutamate elimination. NMR analysis revealed for the first time that the glutamyl group was attached to a serine via an ester bond, by the catalysis of LanB-type enzymes. Our findings provide insights into the function of separated-type LanBs involved in the biosynthesis of goadsporin and thiopeptides.
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