Journal
CHEMBIOCHEM
Volume 17, Issue 12, Pages 1122-1126Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201600076
Keywords
disulfides; DNA structures; dynamic combinatorial chemistry; nucleic acids; reduction
Funding
- Danish National Research Foundation [DNRF81]
- European School of DNA Nanotechnology (EScoDNA), a Marie Curie ITN under FP7
Ask authors/readers for more resources
Designed nanostructures formed by self-assembly of multiple DNA strands suffer from low stability at elevated temperature and under other denaturing conditions. Here, we propose a method for covalent coupling of DNA strands in such structures by the formation of disulfide bonds; this allows disassembly of the structure under reducing conditions. The dynamic chemistry of disulfides and thiols was applied to crosslink DNA strands with terminal disulfide modifications. The formation of disulfide-linked DNA duplexes consisting of three strands is demonstrated, as well as a more-complex DNA double-crossover tile. All the strands in the fully disulfide-linked structures are covalently and geometrically interlocked, and it is demonstrated that the structures are stable under heating and in the presence of denaturants. Such a reversible system can be exploited in applications where higher DNA stability is needed only temporarily, such as delivery of cargoes to cells by DNA nanostructures.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available