Journal
CHEMBIOCHEM
Volume 17, Issue 14, Pages 1367-1373Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201600173
Keywords
assay; bio-electrochemistry electrode; electrochemistry; enzyme catalysis; nanotubes; P450
Funding
- German Ministry of Education and Research [031A179]
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Although electrochemically catalysed P450 reactions have been described, their efficiency and applicability remained limited. This is mostly due to low enzyme activity, laborious protein immobilisation and the small electrode surface. We established a novel protein immobilisation method for a determined orientation and electrical wiring of the enzyme without post-expression modification. By genetic introduction of an anchorpeptide our method is applicable for screening medium to large mutant libraries and detection by an electrode system. The system was expanded by using wired carbon nanotubes within a sol-gel matrix to create a three dimensional electrode.
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