Journal
CHEMBIOCHEM
Volume 17, Issue 6, Pages 479-485Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201500517
Keywords
beta-peptide; membranes; protein-protein interactions; SNARE proteins; transmembrane domains; vesicles
Funding
- Deutsche Forschungsgemeinschaft DFG [SFB 803]
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Artificial SNARE analogues derived from SNARE proteins, which mediate synaptic membrane fusion, are of interest. They mimic the tetrameric -helix bundle of the SNARE motif with various bio-oligomer recognition units. Interaction between complementary oligomers linked to the respective membrane by lipid or peptide anchors leads to proximity of vesicles and to fusion of lipid bilayers. -Peptide nucleic acids were introduced as hybrid oligomers with the native SNARE protein transmembrane/linker sequence, in order to evaluate a fusion system that allows distance tuning of approaching membranes. Formation of a four-base pair -PNA double strand with 20 angstrom length is sufficient for vesicle membrane fusion. Elongation of the recognition -PNA duplex in the linker region yielded a 40 angstrom -peptide duplex and provided a vesicle-vesicle distance that only supported hemifusion of vesicle membranes.
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